phenylmethylsulfonylfluoride

English

Noun

phenylmethylsulfonylfluoride (uncountable)

1. (biochemistry) A serine protease inhibitor commonly used in the preparation of cell lysates.
   • 2015 June 5, Jun Chai et al., “Effects of sevoflurane on tight junction protein expression and PKC-α translocation after pulmonary ischemia–reperfusion injury”, in Experimental & Molecular Medicine‎^[1], volume 47, →DOI:

     One hundred milligrams of frozen lung tissue was homogenized in buffer A (0.32 M sucrose, 5 m M Tris–HCl pH 7.5, 120 m M KCl, 1 m M EDTA, 0.2 m M phenylmethylsulfonylfluoride, 1 μg ml −1 leupeptin, 1 μg ml −1 pepstatin and 1 μg ml −1 aprotinin) and spun at 4 °C, 12 890  g for 10 min.

Synonyms

• PMSF