misprime

English

Etymology

mis- +‎ prime

Verb

misprime (third-person singular simple present misprimes, present participle mispriming, simple past and past participle misprimed)

1. To prime incorrectly.
   • 2007, Ralph Rapley, John M. Walker, Molecular Biomethods Handbook, page 310:

     It is also useful when using degenerate primers which may misprime and cause nonspecific products.

   • 2010, Stephen A. Bustin, The PCR Revolution: Basic Technologies and Applications, page 139:

     As described previously, primers are apt to dimerize or misprime, particularly in the absence of specific target.

   • 2013, Stuart LeGrice, Matthias Gotte, Human Immunodeficiency Virus Reverse Transcriptase, page 208:

     Aberrant annealing has also been observed — either due to the use of the incorrect tRNA to misprime reverse transcription (Colicelli and Goff 1986a, b; Wakefield et al. 1995) or due to the priming of cellular mRNAs with short “tRNA-like” primers (Taylor and Cywinski 1984).